Dr. John Jacob Osterhout
- Postdoctoral: Stanford Medical School (1985â€“88)
- Welch Fellow, (1982â€“85) UTHSC Houston
- Ph.D., (1981) University of Texas at Austin
- B.S., (1974) Rice University
CHEM 1301, CHEM 1411, CHEM 4333, CHEM 6383
My laboratory studies a model peptide, Î±Ï„Î±, which is a de novo designed helix-turn-helix peptide. Circular Dichroism (CD) and Nuclear Magnetic Resonance (NMR) are used to study the conformation of this peptide and its variants. The experimental results are described by the extended helix-coil (XHC) model that is an extension of Lifson-Roig theory. The theory allows us to calculate the balance between helix formation and hydrophobic collapse in protein folding.
My laboratory also studies snake venom and its interaction with antivenom. In an earlier study an undergraduate researcher examined the effects of foaming-induced denaturation on the physical properties and activity of the commercial antivenoms available in North America. My current work concerns the protein components, and their individual and geographic variation in Crotalus atrox (western diamondback rattlesnake) and Agkistrodon piscivorus (Cottonmouth) venoms. I am a member of the VIPER Institute (VIPER = Venom, Immunology, Pharmacology and Emergency Response) in Tucson, Arizona.
- Xian, W., Connolly, P. J., Oslin, M., Hausrath, A. C. & Osterhout, J. J (2006) Fundamental interactions in protein folding: Measuring the energetic balance between helix formation and hydrophobic interactions. Protein Science 15, 2062-2070.
- Kanavage, A. D., Boyer, L. V., McNally, J. & Osterhout, J. J. (2006) Renaturation of antivenom proteins after foaming. Toxicon 47, 445-452.
- Fezoui, Y., Hartley, D. M., Walsh, D. M., Selkoe, D. J., Osterhout, J. J. & Teplow, D. B. (2000) A de novo designed helix-turn-helix peptide forms nontoxic amyloid fibrils. Nat. Struct. Biol. 7, 1095-1099.
- Knubovets, T., Osterhout, J. J., Connolly, P. J. & Klibanov, A. M. (1999) Structure, thermostability and conformational flexibility of hen egg-white lysozyme dissolved in glycerol. Proc. Natl. Acad. Sci. USA 96, 1262-1267.
- Fezoui, Y., Connolly, P. J. & Osterhout, J. J. (1997) Solution structure of Î±Ï„Î±, a helical hairpin peptide of de novo design. Prot. Sci. 6, 1869-1877.
- Fezoui, Y., Weaver, D. L. & Osterhout, J. J. (1995) Strategies and rationales for the de novo design of a helical hairpin peptide. Prot. Sci. 4, 286-295.
Research associate professor, Biochemistry and Molecular Biophysics Department, University of Arizona, Tucson, Ariz. (2002â€“08)
Head of the protein folding and design group, The Rowland Institute for Science, Cambridge, Mass. (1988â€“2001)